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BactFreeze
Freezing Bacterial Stocks. Spin an overnight saturated culture at 2500rpm for 12 min at 4°C. Discard the supernatant. For a 5 ml culture, add 250 ul of sterile 30% glycerol and 250 ul of L broth. ( Add antibiotic, if necessary. ... and tetracycline for preparation of antibitic stocks). Freeze in boxes in ...

Colony Picking and Cell Growth
This is the protocol we uae for picking colonies and growing E. coli cells. ... appropriate antibiotics, proceed to colony picking, preparation of glycerol stocks and the growth of liquid cultures ... to allow sufficient lengths of time to prepare glycerol stocks ...

Open Biosystems: Clone Resources - Yeast Collections - Yeast Knock Outs
... been transferred onto agar directly from the glycerol stocks. Each agar dish is the same ... YSC1053 Yeast MATa Collection - Glycerol Stocks. YSC1063 Yeast MATa Collection - Agar Plates ...

www.merck.co.nz/content_files/Novagen%20glycerol%20promo.pdf
Glycerol Stock Promotion. Novagen®. has discontinued many of its glycerol stocks in the new 2004 catalogue (available April/May), and is offering the remaining stocks specifically to its customers in Asia Pacific for hugely. reduced prices. ... same product, different batches. Glycerol stocks are stable for at least a ...

Procurement and Curation of Genome-scale Nucleic Acid Collections
... Prior to replication of glycerol stocks or propagation of miniprep cultures, we carefully plan our strategy ... To replicate glycerol stocks, source plates are placed in a laminar flow ...